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A full set toolkit for base editing, prime editing, TR-HDR, and targeted gene insertion

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Open Genome: one software for all precision genome editing

Step1

Select reference Genome

Genome Select:

Step2

Input your sequence /(Upload from fasta File)(5'and 3' fragment relative to edited site (>100 bp if possible)

* Input editing formats are the same for all kinds of editing types, with >100 bp of the 5’ and 3’ fragment relative to the edited site being considered.

Substitution

Deletion

Insertion

Step3

Choose editing method

Base editing: (CBE) converts C•G to T•A; (ABE) converts the conversion of A•T to G•C Prime editing: Short sequence insertion or replacement, point mutation of all 12 types of point mutation TR-HDR: Insertion , replacement or deletion for both long and short sequences Targeted Targeted insertion: High efficient sequence insertion of both long and short types at cut site thought NHEJ

Cas types:

Choose Cas types

SpCas9 (Streptococcus pyogenes : 5’-NGG-3’) SpCas9-NG (Rationally engineered SpCas9 variant : 5’-NG-3’) SPRY (Rationally engineered SpCas9 variant : 5’- NRN-3’ and to a lesser extent 5’- NYN-3’) dLbCpf1 (Lachnospiraceae bacterium : 5’- TTTV-3’ ) SACas9

Cas types:

Choose Cas types

Cas types:

Choose Cas types

Cas types:

Choose Cas types

Parameters

Exclude UUUU numbers: PBS+RT templates in total

Exclude first C in RT template

Parameters

Sequence you want to insert:

show NGG

char in Line

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