A full set toolkit for base editing, prime editing, TR-HDR, and targeted gene insertion

OpenGenome project provides a set of tools for the design of target-sgRNAs (targetDesign), prediction of off-target sites (offTarget), design of primers for construction of the sgRNA expression cassettes and amplification of genomic DNA containing target site(s) (primerDesign), determination of mutant sequences from complex sequencing chromatograms of PCR amplicons (DSDecodeM), and download of genomic sequences of certain regions from reference genomes (seqDownload).

Step1

Select reference Genome

Step2

Input your sequence /(Upload from fasta File)(5'and 3' fragment relative to edited site (>100 bp if possible)

* Input editing formats are the same for all kinds of editing types, with >100 bp of the 5’ and 3’ fragment relative to the edited site being considered.

Step3

Edit your sequence as you want

Substitution
|
Deletion
|
Insertion
*Note :Substitution could directedly edit on the sequence without any symbols ;the insertion should use the format as [
aacct
]; the deletion should use the format {
TCCTT
}.All of the precise editing should be lower case letters.

Step4

Choose editing method

Cas types:

Choose Cas types